Fig 1: Statistical analysis of viable and dead cells in controls and VA-treated conditions. Vernonia amygdalina reduces the viability of mouse iPSCs in a concentration-dependent manner. Cells were incubated with increasing concentration of VA (0 to 80 mg/mL) for 24 h. Thereafter, the samples were washed to remove the compound. The cells were then allowed to recover in fresh medium for 2 h. After wise, Trypan blue exclusion method was used to assess cell viability. Suspension of each sample was mixed with 0.4% Trypan blue solution for 10 min and cells were counted in an EVE automatic cell counter (NanoEntek, VWR, Munich, Germany). Experiments were performed at least 3 times and results are presented as the mean ± SEM, and are statistically significant for * p < 0.05.